mouse st2 il 33r duoset elisa kit  (R&D Systems)

Journal: Journal of Histochemistry and Cytochemistry

Article Title: Dynamic Expression of Interleukin-33 and ST2 in the Mouse Reproductive Tract Is Influenced by Superovulation

doi: 10.1369/0022155420911049

Figure Lengend Snippet: Expression of interleukin-33 (IL-33) and its receptor subunit, ST2, in pubescent ovaries and uteri. (A) Representative image of an H&E stained ovary. Asterisks identify oocytes in secondary follicles. Arrows identify oocytes in antral follicles. (B–F) Representative images of immunostained ovaries. (B, D, E) CD31 expression identifies endothelial cells associated with vasculature in the ovary. (C, D, F) IL-33 is expressed in oocytes of primordial (asterisk in D), secondary (asterisk in inset of C; asterisk in F) and antral follicles (C, arrow). (E, F) ST2 is expressed in granulosa cells (gc) of follicles and in the stroma (st). (G, H) Representative images of immunostained uteri. (G) Pan-cytokeratin (cytok) expression identifies glandular (yellow arrowheads) and luminal (white arrowheads) epithelia. IL-33 is detected in the myometrium (white arrow). A few IL-33+ cells are detected in the luminal epithelium (yellow box, inset). (H) CD31 expression identifies endothelial cells associated with vasculature in the uterine stroma and myometrium. ST2 is expressed in glandular (yellow arrowheads) and luminal (white arrowheads) epithelia. ST2 is detected in the myometrium (white arrow). Abbreviation: HE, hematoxylin & eosin; Bars = 500 µm (A–C, G), 50 µm (D), and 100 µm (E, F, H).

Article Snippet: Serum ST2 levels were quantified using a ST2 (IL-33R) mouse ELISA Kit (ThermoFisher Scientific).

Techniques: Expressing, Staining

Journal: Journal of Histochemistry and Cytochemistry

Article Title: Dynamic Expression of Interleukin-33 and ST2 in the Mouse Reproductive Tract Is Influenced by Superovulation

doi: 10.1369/0022155420911049

Figure Lengend Snippet: Summary of IL-33 and ST2 Expression in Pubescent and Sexually Mature Ovaries and Uteri.

Article Snippet: Serum ST2 levels were quantified using a ST2 (IL-33R) mouse ELISA Kit (ThermoFisher Scientific).

Techniques: Expressing

Journal: Journal of Histochemistry and Cytochemistry

Article Title: Dynamic Expression of Interleukin-33 and ST2 in the Mouse Reproductive Tract Is Influenced by Superovulation

doi: 10.1369/0022155420911049

Figure Lengend Snippet: Expression of ST2 and sST2, the decoy receptor for IL-33, in estrus and diestrus-stage ovaries. (A–C) Representative images of immunostained ovaries. (A) In diestrus, ST2 is expressed in granulosa cells of secondary follicles (A, arrows). In estrus, ST2 is expressed in stroma (st) and in granulosa cells of primary follicles (B, yellow arrow) and antral follicles (B, arrowhead). (C) In estrus, ST2 is expressed in luteinized granulosa cells and in the theca layer (between dashed lines) of corpora lutea (CL). (D) qRT-PCR on RNA extracted from ovarian homogenates demonstrate decreased sST2 expression in diestrus as compared with estrus. Data are shown for each individual animal analyzed (n=4–5 animals) with median and interquartile range. Abbreviations: sST2, soluble form of ST2; IL, interleukin; RNA, ribonucleic acid; qRT-PCR, quantitative RT-PCR. Bars = 100 µm (A–C). *p<0.05. Asterisks identify oocytes in A and B.

Article Snippet: Serum ST2 levels were quantified using a ST2 (IL-33R) mouse ELISA Kit (ThermoFisher Scientific).

Techniques: Expressing, Quantitative RT-PCR

Journal: Journal of Histochemistry and Cytochemistry

Article Title: Dynamic Expression of Interleukin-33 and ST2 in the Mouse Reproductive Tract Is Influenced by Superovulation

doi: 10.1369/0022155420911049

Figure Lengend Snippet: Estrous-stage alters IL-33, but not ST2 expression in uteri. (A–H) Representative images of immunostained uteri. A diestrus-stage uterine section showing pan-cytokeratin expression (A) in glandular (arrow) and luminal (arrowheads) epithelia and CD31 (A, D) expression in endothelial cells. In estrus, IL-33 (B) and ST2 (E) are expressed in luminal (white arrowheads) and glandular (white arrows) epithelia. The inset in B shows expression of IL-33 (green) and pan-cytokeratin (red) in an estrus-stage lumen. In diestrus, IL-33 (C) is not expressed in luminal epithelia (white arrowheads) but is increased in the myometrium (m). The inset in C is a diestrus lumen showing pan-cytokeratin (red) and lack of expression of IL-33 (green). In diestrus, ST2 (F) is expressed in luminal (white arrowheads) and glandular (white arrows) epithelia. Magnified images of lumen (G) and glands (H) highlight IL-33 and ST2 expression. To calculate the percentage of IL-33+ luminal cells, the ratio of IL-33+ cells to the total number of DAPI+ cells in 0.23 mm2 lumen was determined. To calculate the percentage of IL-33+ cells in the glandular epithelium, the total number of IL-33+ cells in 100–150 glandular cells was determined. (I) Compared with estrus, IL-33 expression during diestrus was lower in luminal but not glandular epithelium. Data are shown for each individual animal analyzed (n=4 animals) with median and interquartile range. Abbreviations: IL, interleukin; DAPI, 4′,6-diamidino-2-phenylindole. *p<0.05. Bars = 50 µm (A–G).

Article Snippet: Serum ST2 levels were quantified using a ST2 (IL-33R) mouse ELISA Kit (ThermoFisher Scientific).

Techniques: Expressing

Journal: Journal of Histochemistry and Cytochemistry

Article Title: Dynamic Expression of Interleukin-33 and ST2 in the Mouse Reproductive Tract Is Influenced by Superovulation

doi: 10.1369/0022155420911049

Figure Lengend Snippet: Expression of IL-33 and ST2 in ovaries and uteri in early pregnancy. (A–C) Representative images of immunostained ovaries. (A–C) At E0.5, IL-33 is expressed in the stroma (st) and in the theca layer surrounding follicles and corpora lutea. Cells expressing IL-33 are adjacent to endothelial cells expressing CD31 (white boxes in B). (C) IL-33 is expressed in oocytes of primordial follicles (arrows) and in oocytes (asterisk) of secondary follicles (arrowhead). ST2 is expressed in granulosa cells and in the stroma (st). (D–G) Representative images of immunostained uteri in early pregnancy. Pan-cytokeratin (cytok) expression identifies glandular (arrows) and luminal (white arrowheads) epithelia. (D) At E0.5, IL-33 is detected in the myometrium (m) and in the glandular (arrows) and luminal (arrowheads) epithelium. (E) At E0.5, ST2 is expressed in the luminal (arrowheads) and glandular (arrows) epithelia. (F) At E2.5, co-staining of uteri with epithelial markers, pan-cytokeratin (cytok), and E-cadherin (E-cad) shows ST2 expression in all luminal epithelium and IL-33 in a subset of those cells. (G) At E2.5, co-staining of the uteri with glandular marker FoxA2 and E-cadherin shows ST2 expression in all glandular epithelial cells and IL-33 in a subset of those cells. Abbreviation: IL, interleukin; Bars = 500 µm (A), 100 µm (B, C) and 50 µm (D–G).

Article Snippet: Serum ST2 levels were quantified using a ST2 (IL-33R) mouse ELISA Kit (ThermoFisher Scientific).

Techniques: Expressing, Staining, Marker

Journal: Journal of Histochemistry and Cytochemistry

Article Title: Dynamic Expression of Interleukin-33 and ST2 in the Mouse Reproductive Tract Is Influenced by Superovulation

doi: 10.1369/0022155420911049

Figure Lengend Snippet: Superovulation increases local IL-33 and sST2 levels. (A–B) qRT-PCR analysis of ovarian and uterine gene expression at E0.5 and E2.5 in natural mating cycles. (A) Relative to E0.5, ovarian expression of IL-33 at E2.5 was significantly decreased. Ovarian expression of sST2, StAR, and VEGFa was not statistically different at E2.5 as compared with E0.5. (B) Uterine expression of Il33 mRNA was similar at E0.5 and E2.5. Relative to E0.5, uterine expression of progesterone receptor (PR) at E2.5 was significantly increased. (C–D) qRT-PCR analysis of ovarian and uterine gene expression at E0.5 in natural mating cycles as compared with E0.5 in SO cycles. (C) Relative to NCs, ovarian expression of transcripts for IL-33, sST2, StAR, and VEGFa was increased in SO cycles. (D) Uterine expression of Il33 and PR was increased in SO cycles as compared with NCs. For all qRT-PCR, expression levels were normalized to 18s rRNA. Data are shown for each individual animal analyzed (n=4–8 animals) with median and interquartile range. Abbreviations: IL, interleukin; sST2, soluble form of ST2; qRT-PCR, quantitative RT-PCR; StAR, steroidogenic acute regulatory protein; VEGFa, vascular endothelial growth factor A; SO, superovulation; NC, natural cycle; rRNA, ribosomal ribonucleic acid. *p<0.05, **p<0.01, ***p<0.001.

Article Snippet: Serum ST2 levels were quantified using a ST2 (IL-33R) mouse ELISA Kit (ThermoFisher Scientific).

Techniques: Quantitative RT-PCR, Gene Expression, Expressing

Journal: Journal of Histochemistry and Cytochemistry

Article Title: Dynamic Expression of Interleukin-33 and ST2 in the Mouse Reproductive Tract Is Influenced by Superovulation

doi: 10.1369/0022155420911049

Figure Lengend Snippet: Superovulation does not alter circulating IL-33 and sST2 levels. Circulating levels of ST2 and progesterone were determined by ELISA and immunoassay, respectively. (A) Serum levels of ST2 were similar at E0.5 and E2.5. Serum progesterone levels were higher at E2.5 as compared with E0.5. (B) Serum levels of ST2 were similar in NCs and SO cycles at E0.5. Serum progesterone levels were higher in SO cycles as compared with NCs. Data are shown for each individual animal analyzed (n=4–7 animals) with median and interquartile range. Abbreviations: IL, interleukin; sST2, soluble form of ST2; ELISA, enzyme-linked immunosorbent assay; NC, natural cycle; SO, superovulation. **p<0.01, ***p<0.001.

Article Snippet: Serum ST2 levels were quantified using a ST2 (IL-33R) mouse ELISA Kit (ThermoFisher Scientific).

Techniques: Enzyme-linked Immunosorbent Assay